RESUMEN
Characterizing the nutritional needs of wild bee species is an essential step to better understanding bee biology and providing suitable supplemental forage for at-risk species. Here, we aim to characterize the nutritional needs of a model solitary bee species, Osmia cornifrons (Radoszkowski), by using dietary protein-to-lipid ratio (P:L ratio) as a proxy for nutritional niche and niche breadth. We first identified the mean target P:L ratio (~3.02:1) and P:L collection range (0.75-6.26:1) from pollen provisions collected across a variety of sites and time points. We then investigated the P:L tolerance range of larvae by rearing bees in vitro on a variety of diets. Multifloral and single-source pollen diets with P:L ratios within the range of surveyed provisions did not always support larval development, indicating that other dietary components such as plant secondary compounds and micronutrients must also be considered in bee nutritional experiments. Finally, we used pollen metabarcoding to identify pollen from whole larval provisions to understand how much pollen bees used from plants outside of their host plant families to meet their nutritional needs, as well as pollen from individual forager bouts, to observe if bees maintained strict floral constancy or visited multiple plant genera per foraging bout. Whole larval provision surveys revealed a surprising range of host plant pollen use, ranging from ~5% to 70% of host plant pollen per provision. Samples from individual foraging trips contained pollen from multiple genera, suggesting that bees are using some form of foraging decision making. Overall, these results suggest that O. cornifrons have a wide nutritional niche breadth, but while pollen P:L ratio tolerance is broad, a tolerable P:L ratio alone is not enough to create a quality diet for O. cornifrons, and the plant species that make up these diets must also be carefully considered.
RESUMEN
In honey bees (Apis mellifera), there is growing evidence that the impacts of multiple stressors can be mitigated by quality nutrition. Pollen, which is the primary source of protein and lipids in bees diets, is particularly critical for generating more resilient phenotypes. Here, we evaluate the relationship between pollen protein-to-lipid ratios (P:Ls) and honey bee insecticide resilience. We hypothesized that pollen diets richer in lipids would lead to increased survival in bees exposed to insecticides, as pollen-derived lipids have previously been shown to improve bee resilience to pathogens and parasites. Furthermore, lipid metabolic processes are altered in bees exposed to insecticides.We fed age-matched bees pollen diets of different P:Ls by altering a base pollen by either adding protein (casein powder) or lipids (canola oil) and simulating chronic insecticide exposure by feeding bees an organophosphate (Chlorpyrifos). We also tested pollen diets of naturally different P:Ls to determine if results are consistent. Linear regression analysis revealed that mean survival time for altered diets was best explained by protein concentration (p =0.04 , adjusted R2 =0.92), and that mean survival time for natural diets was best explained by P:L ratio (p =0.008 , adjusted R2 =0.93). Our results indicate that higher ratios of dietary protein to lipid has a negative effect on bee physiology when combined with insecticide exposure, while lower ratios have a positive effect. These results suggest that protein and lipid intake differentially influence insecticide response in bees, laying the groundwork for future studies of metabolic processes and development of improved diets.
RESUMEN
Pollinator nutritional ecology provides insights into plant-pollinator interactions, coevolution, and the restoration of declining pollinator populations. Bees obtain their protein and lipid nutrient intake from pollen, which is essential for larval growth and development as well as adult health and reproduction. Our previous research revealed that pollen protein to lipid ratios (P:L) shape bumble bee foraging preferences among pollen host-plant species, and these preferred ratios link to bumble bee colony health and fitness. Yet, we are still in the early stages of integrating data on P:L ratios across plant and bee species. Here, using a standard laboratory protocol, we present over 80 plant species' protein and lipid concentrations and P:L values, and we evaluate the P:L ratios of pollen collected by three bee species. We discuss the general phylogenetic, phenotypic, behavioral, and ecological trends observed in these P:L ratios that may drive plant-pollinator interactions; we also present future research questions to further strengthen the field of pollination nutritional ecology. This dataset provides a foundation for researchers studying the nutritional drivers of plant-pollinator interactions as well as for stakeholders developing planting schemes to best support pollinators.
